By Annick Wilmotte (auth.), Donald A. Bryant (eds.)
More than 20 years in the past, as a fledgling graduate a few abnormal features of the genetics of those scholar who used to be simply commencing to know about those organisms yet to pay respects to the 2 volumes of organisms that will turn into my fundamental study Carr of Whitton that performed very important roles in my concentration, the ebook of Noel Carr and Brian personal pondering cyanobacteria (and without doubt in Whitton's The Biology of the Blue-Green Algae within the improvement of many others as well). Contri 1973 was once an occasion of serious importance. till the buting authors have been requested to explain not just what visual appeal of this treatise, there has been no unmarried quantity we all know at the moment, but in addition to indicate issues we on hand that awarded a large evaluate of the have no idea but. i've got tried to gather a booklet biology and biochemistry ofthese organisms. approximately that may stimulate graduate scholars and different ten years later, i used to be privileged to be a contributing researchers within the related approach that i used to be plagued by the writer to Carr and Whitton's sequel quantity The books pointed out above. Biology of the Cyanobacteria. even supposing the it seems that cyanobacterial molecular biologists intervening interval have been marked via heated debates have certainly paid consciousness to the admonition in their over the taxonomy and taxonomic place of the erstwhile colleague, W Ford Doolittle, to 'study organisms, it used to be additionally a time while the comparative these issues that cyanobacteria do well.
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Additional info for The Molecular Biology of Cyanobacteria
To check for the possibility of gene transfer, sequence data from one or two other genes should be compared with the results obtained by means of the 16S rRNA sequences. • According to Woese (1987), the 16S rRNA is a good 'molecular chronometer' -that is, it is a good molecule for measuring the overall rate of evolutionary change in a line of descent. , 1993). , 1993) are used for sequencing using the dideoxynucleotide method. In addition to axenic cultures, cyanobacterial strains contaminated by other bacteria can also be used, after design of peR primers allowing a selective amplification of the cyanobacterial sequence.
Other genera of section II should be investigated. , 1992), are unstable and their relationships are still uncertain. New sequences from closely related strains should provide useful information. The strain Chamaesiphon sp. strain pee 7430 is unicellular and has the peculiar ability to reproduce by asymmetric fission or budding. , 1988). To ascertain whether this type of division is a good phylogenetic marker, the 16S rRNA sequences of other Chamaesiphon sp. strains should be determined. g. 6%).
In addition, the length variability of the ITS sequence may allow the detection of the presence of more than one cyanobacteria in a culture. , 1992). The only cyanobacterial23S rRNA sequence available is that from Synechococcus sp. ,1983). Finally, for a rapid taxonomic survey, amplified portions of the rRNA cistrons may be digested by restriction enzymes. , 1992). VI. Conclusion This chapter has underlined the problems addressed by the evolutionary and taxonomic studies of cyanobacteria, and presented the promising tools offered by molecular techniques.