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This booklet constitutes the completely refereed post-conference complaints of the 14th overseas assembly on DNA Computing, DNA 14, held in Prague, Czech Republic, in June 2008.
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Additional info for DNA Computing: 14th International Meeting on DNA Computing, DNA 14, Prague, Czech Republic, June 2-9, 2008. Revised Selected Papers
6. : From Genes to Machines: DNA Nanomechanical Devices. Trends Biochem. Sci. 30, 119–125 (2005) 7. : DNA-Based Machines. Org. Biomol. Chem. 4, 3392–3401 (2006) 8. : A DNA-Fuelled Molecular Machine Made of DNA. Nature 406, 605–608 (2000) 9. : A Modular DNA Signal Translator for the Controlled Release of a Protein by an Aptamer. Nucleic Acid Res. 34, 1581–1587 (2006) 10. : A Synthetic DNA Walker for Molecular Transport. J. Am. Chem. Soc. 126, 10834–10835 (2004) 11. : Molecular Gears: A Pair of DNA Circles Continuously Rolls against Each Other.
5). On the contrary, the fluorescence became lower after 5 min of UV light irradiation, although the fluorescence was still much higher than that of the completely closed state (compare blue line with green line ---- in Fig. 5). It can be estimated that about 13% of the tweezers were photoregulated to open and close. This lower efficiency of closing the tweezers may be attributed to the insufficient stabilization effect in cis form as we reported recently . When visible light was irradiated for 1 min at this point, the fluorescence increased and the closed tweezers opened again.
The opening and closing are photoregulated to some extent with UV (green lines ----) and visible light (blue lines ⋅⋅⋅⋅) irradiation, respectively. The efficiency for the photoregulation of closing and opening of the tweezers with F12X was estimated by measuring the change of fluorescence with the light irradiation at thermo-stable conditions. As shown in Fig. G. Liang et al. (closed tweezers). On the other hand, when it was irradiated with UV light (330~350 nm) for 5 minutes, the spectra (green lines ----) became close to that of the opening tweezers consisting of only A, B, and C (Fig.