By Rocky S. Tuan (Editor), Cecilia W. Lo (Editor)
Drawing on state of the art mobile and molecular recommendations in addition to new and complicated imaging and knowledge applied sciences, this entire, three-volume number of state of the art protocols presents conveniently reproducible tools for learning and studying the occasions of embryonic improvement. quantity 1 (ISBN: 089603-574-3) comprises ideas for constructing and characterizing numerous primary experimental version structures, for the examine of developmental styles and morphogenesis, and for the exam of embryo constitution and serve as. There also are step by step equipment for the analaysis of mobile lineage, the creation and use of chimeras, and the experimental and molecular manipulation of embryos, together with the appliance of viral vectors. quantity 2 (ISBN: 0-89603-575-1) describes state of the art tools for the research of organogenesis, the research of irregular improvement and teratology, the screening and mapping of novel genes and mutations, and the appliance of transgenesis, together with the construction of transgenic animals and gene knockouts. No much less leading edge, quantity three (ISBN: 0-89603-576-X) introduces robust ideas for the manipulation of developmental gene expression and serve as, the research of gene expression, the characterization of tissue morphogenesis and improvement, the in vitro research of differentiation and improvement, and the genetic research of developmental types of illnesses. hugely sensible and richly annotated, the 3 volumes of Developmental Biology Protocols describe a number of experimental platforms and information innovations followed from the broadest array of biomedical disciplines.
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Additional info for Developmental Biology Protocols, Volume II (Methods in Molecular Biology Vol 136)
3) from transformed Xenopus fibroblasts. ” Activin is a dimeric protein and has been described in mammals as sharing subunits with a related dimeric protein—inhibin (Fig. 1). Inhibin is another peptide, which, in contrast to activin, inhibits the secretion of FSH by pituitary cells. Although inhibins have been identified in mammals, they have not yet been described in amphibia. Activin warrants nomination as a candidate endogenous inducer, for it is found in embryos at the expected place and at the appropriate time to play a role in mesoderm formation.
A. (1992) Embryonic expression and functional analysis of a Xenopus activin receptor. Dev. Dyn. 194, 1–11. Armes, N. A. and Smith, J. C. (1997) The ALK-2 and ALK-4 activin receptors transduce distinct mesoderm-inducing signals during early Xenopus development but do not cooperate to establish thresholds. Development 124, 3797–3804. , and Melton, D. A. (1994) Inhibition of activin receptor signaling promotes neuralization in Xenopus. Cell 77, 273–281. New, H. , Kavka, A. , Smith, J. , and Green, J.
Melby, T. , and McCartney, B. M. (1997) Isolation of mutations in the Drosophila homologues of the human Neurofibromatosis 2 and yeast CDC42 genes using a simple and efficient reverse-genetic method. Genetics 146, 245–252. 26. Brand, A. H. and Perrimon, N. (1993) Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development 118, 401–415. 27. Bishop, J. , III and Corces, V. (1988) Expression of an activated ras gene causes developmental abnormalities in transgenic Drosophila melanogaster.