By Martin Egli, Piet Herdewijn, Akira Matusda
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1985. Fe(II). Proc. Natl. Acad. Sci. A. 82:968-972. J. J. 1987. Synthesis and application of derivatizable oligonucleotides. Nucl. Acids Res. 15:6455-6467. Goodchild, J. 1990. Conjugates of oligonucleotides and modified oligonucleotides: A review of their synthesis and properties. Bioconjugate Chem. 1:165-187. E. 1995. Synthesis and characterization of fluorescent oligonucleotides. Effect of internal labelling on protein recognition. Biochim. Biophys. Acta 1244:259-268. W. Jr. 1989. Palladium-catalyzed synthesis of alkynylamino nucleosides.
L. 1977. Preparation of C-5 mercurated pyrimidine nucleosides. J. Carbohydrates Nucleotides Nucleosides 42:257269. , and Lin, X. 1991. Palladium-mediated synthesis of C-5 pyrimidine nucleoside thioethers from disulfides and mercurinucleosides. J. Org. Chem. 56:5598-5602. C. T. 1995. Very high affinity DNA recognition by bicyclic and crosslinked oligonucleotides. J. Am. Chem. Soc. 117:10434-10442. L. 1988. Synthesis and hybridization of a series of biotinylated oligonucleotides. Nucl. Acids Res.
1 filter paper and a Buchner funnel. 11. Rinse the precipitate with cold acetonitrile (4°C) and allow the crystals to air dry. 12. Recrystallize from a minimum amount of hot methanol. Place the solid in a 500-mL Erlenmeyer flask and add a few milliliters of methanol. Heat with gentle shaking on a hot plate or in a steam bath until boiling. Gradually add methanol until all the solid is dissolved. Remove from heat and allow to cool. Recrystallization can easily be carried out on a larger scale. 13.