Download Culture of Human Stem Cells (Culture of Specialized Cells) by R. Ian Freshney, Glyn N. Stacey, Jonathan M. Auerbach PDF

By R. Ian Freshney, Glyn N. Stacey, Jonathan M. Auerbach

This book collects the best and state of the art equipment and protocols for deriving and culturing human embryonic and grownup stem cells—in one convenient resource.
This groundbreaking book follows the culture of earlier books within the tradition of specialised Cells Series—each tools and protocols bankruptcy is laid out precisely just like the subsequent, with stepwise protocols, preceded by way of particular necessities for that protocol, and a concise dialogue of equipment illustrated by way of information. The editors describe a constrained variety of consultant options throughout a large spectrum of stem cells from embryonic, infant, and grownup tissue, yielding an all-encompassing and flexible advisor to the sphere of stem cellphone biology and culture.
The publication incorporates a finished checklist of providers for all gear utilized in the protocols offered, with web content on hand in an appendix. also, there's a bankruptcy on qc, and different chapters overlaying felony and moral matters, cryopreservation, and feeder layer culture. This textual content is a one-stop source for all researchers, scientific scientists, academics, and scholars interested by this significant zone of analysis.

Show description

Read or Download Culture of Human Stem Cells (Culture of Specialized Cells) PDF

Best biology books

Biology of Copper Complexes

In 1928, it used to be stumbled on that copper was once crucial for regular human metabolism. Ten years later, 1938, it was once saw that sufferers with rheumatoid arthritis had a better than general serum copper focus, which lower back to basic wi th remission of this ailment. 13 years later, it was once chanced on that copper complexes have been powerful in treating arthritic ailments.

Molecular and Cellular Biology of Multidrug Resistance in Tumor Cells

The power of neoplastic cells to outlive publicity to numerous chemotherapeutic medicinal drugs represents the most hindrance to profitable melanoma chemotherapy. This booklet offers with a specific form of resistance in tumor cells that represents a unmarried yet specifically vital element of the multifaceted challenge of melanoma drug resistance.

Advances in Molecular Biology and Targeted Treatment for AIDS

Because the discovery of HIV-l because the etiologic agent of obtained immunodeficiency syndrome (AIDS) within the early Eighties, amazing growth has been made in either the fundamental figuring out of the organic procedures resulting in AIDS and an sped up attempt find new remedies. As is frequently the case in swiftly advancing fields, lots of the medical discussions are top dealt with in really expert teams.

Extra info for Culture of Human Stem Cells (Culture of Specialized Cells)

Sample text

Check for pH change in broth and subculture if altered. Observe all plates and return to incubation conditions with final observation at 28 days for each plate. Day 42 5. Observe any remaining plates for colonies. Positive cultures should be discarded along with any media and reagents used for the affected cultures. It is possible to eliminate mycoplasma from cell lines with certain antibiotics [Uphoff and Drexler, 2004]. However, success rates for complete eradication are low and the toxic effects of the antibiotics used may alter the characteristics of the cell line.

1. hES-HEPES Medium . . . . . . . . . . . . . . . . . . . . . . . . . . 2. 2 M . . . . . . . . . . . . . . . . . . . . . . . . 3. 1 M . . . . . . . . . . . . . . . . . . . . . . . . 4. Vitrification Medium, 10% . . . . . . . . . . . . . . . . . . . . . . . 5. Vitrification Medium, 20% . . . . . . . . . . . . . . . . . . . . . . . 3. Mouse Embryonic Feeder Cell Medium (MEFM) .

1. Derivation and Cultivation of Human ES Cells . . . . . . . . . . . . . . . . . 2. Propagation of Human ES Cells. . . . . . . . . . . . . . . . . . . . . . . . . 3. Differentiation of Human Embryonic Stem Cells . . . . . . . . . . . . . . . . 2. Cell Culture Medium Components. . . . . . . . . . . . . . . . . . . . . . . . . . . 1. Human Embryonic Stem (hES) Cell Medium . . . .

Download PDF sample

Rated 4.75 of 5 – based on 27 votes