Download Clostridium difficile: Methods and Protocols by Peter Mullany, Adam P. Roberts PDF

By Peter Mullany, Adam P. Roberts

Clostridium difficile, an incredible nosocomial pathogen proven to be a main reason for antibiotic-associated disorder, has emerged as a hugely transmissible and often antibiotic-resistant organism, inflicting a substantial burden on health and wellbeing care platforms all over the world. In Clostridium difficile: equipment and Protocols, specialist researchers compile the main lately constructed equipment for learning the organism, together with options regarding isolation, molecular typing, genomics, genetic manipulation, and using animal versions. Written within the hugely profitable Methods in Molecular Biology™ sequence structure, chapters comprise short introductions to their respective subject matters, lists of the required fabrics and reagents, step by step, effortlessly reproducible laboratory protocols, and notes highlighting pointers on troubleshooting and fending off identified pitfalls.

Authoritative and state of the art, Clostridium difficile: equipment and Protocols serves as an excellent advisor for scientists now capable of achieve an in-depth figuring out of the way this organism is transmitted and the way it factors disease.

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Extra info for Clostridium difficile: Methods and Protocols

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Borriello SP and Barclay FE. (1986) An in-vitro model of colonisation resistance to Clostridium difficile infection. J Med Microbiol 21, 299–309. 53. Aas J, Gessert CE and Bakken JS. (2003) Recurrent Clostridium difficile colitis: case series involving 18 patients treated with donor stool administered via a nasogastric tube. Clin Infect Dis 36, 580–585. 54. Tvede M and Rask-Madsen J. (1989) Bacteriotherapy for chronic relapsing Clostridium difficile diarrhoea in six patients. Lancet 1, 1156–1160.

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2000) Epidemiology of recurrences or reinfections of Clostridium difficile-associated diarrhea. J Clin Microbiol 38, 2386–2388. Hamilton-Miller JM. (1997) Living in the ‘post-antibiotic era’: could the use of probiotics be an effective strategy? Clin Microbiol Infect 3, 2–3. McFarland LV, Surawicz CM, Greenberg RN, et al. (1994) A randomized placebocontrolled trial of Saccharomyces boulardii in combination with standard antibiotics for Clostridium difficile disease. J Am Med Assoc 271, 1913–1918.

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