By A. C. Nag (auth.), Prof. Dr. Dr. H. M. Piper (eds.)
In the cardiovascular sciences, an expanding call for for using sleek equipment of telephone biology has built. using particular cellphone tradition types of many of the tissues concerned is key for many of those novel techniques. This ebook meets the call for for acomprehensive and simple available resource for cellphone cul- ture equipment in cardiovascular examine because it was once no longer formerly on hand. the elemental equipment for cultures of cardiomyocytes (embryonic and adult), endothelial cells (micro- and macrovascular), soft muscle cells and pericytes are defined intimately via a world collection of specialists. targeted chapters speak about using development elements and attachment substrates, concepts for co-cultures, cultures on permeable clear out membranes and microcarrier cultures. The methodological descriptions are sufficiently exact for a right away program within the laboratory. All chapters additionally comprise a serious review of other approaches.
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Additional info for Cell Culture Techniques in Heart and Vessel Research
Mersel M, Hietter H, Luu B (1987) Differential sensitivity of heart fibroblasts and myocytes to 17-fJ-hydroxycholesterol. In: Pinson A (ed) The heart cell in culture, vol 3. CRC, Boca Raton, pp 125-132 55. Mohamed SNW, Holmes R, Hartzell CR (1983) A serum-free chemically defined medium for function and growth of primary neonatal rat heart cell cultures. In Vitro 19:471478 56. Moscona A (1952) Cell suspension from organ rudiments of chick embryos. Exp Cell Res 3: 535-539 57. Nag AC, Cheng M (1984) Expression in cardiac myosin isozymes in cardiac muscle cells in culture.
7 G. Filter the material through the nylon mesh and divide into two 50-ml centrifuge tubes. Spin tubes at 25 g for 3 min. Discard supernatant and resuspend pellet in 50-ml KB medium at room temperature (20°C) in a 250-ml Erlenmeyer flask. Keep at room temperature for at least 45 min. 8G. Resuspend cells every 5 min by gentle agitation of the Erlenmeyer flask. After 45 min, divide suspension into two 50-ml tubes, centrifuge at 25 g for 5 min. Add 500 III Ca stock to 100 ml buffer 1 (37°C). After removal of the supernatant 40 ml of this buffer is added to each tube.
Cell Division Myocardial cells may be distinguished from NMCs in culture by their "dense" cytoplasm, well-developed mitochondria and Golgi system, the presence of myofibrils and intercalated discs, and their spontaneous contraction activity. 5-fold longer in myocardial cells than in NMCs [10, 37]. The general rule that differentiated cells no longer divide has limited applicability to myocardial cells, since the cardiomyoblast, which can synthesize myosin and also divide, is an intermediate state between the presumptive myoblast and the adult postmitotic cardiomyocyte.