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By Renato Baserga, Christian Sell, Mitsuo Yoshinouchi (auth.), Melvin S. Oka Ph.D., Randall G. Rupp Ph.D. (eds.)

Cell Biology and Biotechnology: Novel techniques to Increased mobile Productivity comprises the lawsuits of the symposium via an identical identify held in Cambridge, Massachusetts, January 30 - February 2, 1992. state of the art examine is gifted on: the IGF-1 Receptor and Gene Expression throughout the mobilephone Cycle; Attachment keep an eye on of Fibroplast Proliferation; mobile Biology and Serum-Free Mouse Embryo Cells; Immunoglobulin construction Stimulating elements; Erythropoietin regulate of Programmed dying in Erythroid Progenitors; Prohormone Processing Enzymes and Protein creation; keep an eye on of Translation Initiation via Phosphorylation; Protein Retention within the Endoplasmic Reticulum Mediated through GPR78; Molecular techniques in the direction of Manipulating the Expression of the Glucose comparable Proteins in Mammalian Cells; Protein Folding within the Endoplasmic Reticulum; Sorting of Membrane Proteins within the Endocytic and Exocytic Pathways; CIS-Acting components Which keep watch over Immunoglobulin Gene Transcription.

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13. Guadagno TM, Assoian RK. GlIS control of anchorage-independent growth in the fibroblast cell cycle. 1 Cell Bioi 1991;115:1419-25. 14. Dalton SL, Marcantonio EE, Assoian RK. Cell attachment controls fibronectin and a5~1 integrin levels in fibroblasts: implications for anchoragedependent and -independent growth. 1 Bioi Chern 1992;267:8186-91. 15. Assoian RK. Biphasic effects of type beta transforming growth factor on epidermal growth factor receptors in NRK fibroblasts: functional consequences for epidermal growth factor-stimulated mitosis.

5 x 106 cells and lO-llg aliquots were fractionated on denaturing-agarose gels prior to Northern blot hybridization with probes for histone H4 (A), thymidine kinase (TK) (B), gamma-actin (C), or GAPDH (D). Panels E and F show ethidium-bromide-stained rRNAs. Panels A, C, and E and B, D, and F show results from duplicate filters of the same experiment. Numbers on panels A through D identify the size of each transcript in kilobases (kb). 4 X lO s cpm, respectively. Cell viability in all cultures was 90% (by trypan blue exclusion) throughout the experiment.

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