Download Calcium Handling in hiPSC-Derived Cardiomyocytes by Lee Yee-Ki, Siu Chung-Wah PDF

By Lee Yee-Ki, Siu Chung-Wah

Calcium is essential in governing contractile actions of myofilaments in cardiomyocytes, any defeats in calcium homeostasis of the cells may adversely impact middle pumping motion. The characterization of calcium dealing with houses in human precipitated pluripotent stem cell-derived cardiomyocytes (iPS-CMCs) is of vital curiosity and pertinent to the stem telephone and cardiac regenerative box as a result of their power patient-specific healing use.

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2006), plays a role in enhancing cardiac maturation (Ng et al. 2011). In comparison with the green fluorescent protein (GFP)-transduced control, exogenous apoA-I expression improves calcium homeostasis of human iPSC-derived cardiomyocytes, which exhibited more mature calcium handling properties including larger amplitude, higher maximal upstroke velocity, and maximal decay velocity of a spontaneous calcium transient. To further assess the SR function, application of 10 mM ryanodine, a RyR blocker, substantially reduced the amplitude and upstroke values (approximately 60% reduction compared with drug-free recordings) of spontaneous calcium transients in cardiomyocytes derived from the LV-apoA-I transduced group.

2010, 2011b; Ng et al. 2010). First, according to our experience with murine ESC, T3 supplementation upon cardiac differentiation favorably altered the calcium handling properties of ESCderived cardiomyocytes, including larger calcium transients and faster rate of rise and decay of calcium oscillation. In addition, these cardiomyocytes also appeared to have a larger internal store of calcium as evidenced by the larger amplitude of caffeine-mediated calcium release. These phenotypic changes were likely associated with transcriptional upregulation of calcium handling proteins (RyR2, NCX-1, and SERCA-2a) (Lee et al.

In a t-tubule-rich cell, Ca2+ influx from the cell periphery releases SR stores and then diffuses to the cell interior, which produces a homogeneous calcium spark (Louch et al. 2004; Lieu et al. 2009). The functional consequences of the structural advance will be accessed by line-scan imaging, which reveals temporal and spatial properties of cellular Ca2+ flux in cardiomyocytes. Calcium imaging was performed with Fluo-3 instead of ratiometric calcium indicators such as Indo-1 or Fura-2 because of the high sensitivity.

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