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By Thomas Scheper, P. Bajpai, P.K. Bajpai, D. Dochain, N.N. Dutta, A.C. Ghosh, R.K. Mathur, A. Mukhopadhyay, M. Perrier, P.L. Rogers, H.S. Shin, B. Wang

This quantity bargains with such issues as realities and tendencies in enzymatic prebleaching of Kraft pulp, biotransformation for l-ephedrine construction, inclusion our bodies and purification of proteins in biologically energetic kinds, and extraction and purification of cephalosporin antibiotics.

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1 Pyruvate Decarboxylase (PDC) Activity . . . . . . . . . . . . . . . 2 Metabolic Status of Yeast . . . . . . . . . . . . . . . . . . . 3 Benzaldehyde Concentration . . . . . . . . . . . . . . . . . . 4 End-product Inhibition . . . . . . . . . . . . . . . . . . . . 3 Biotransformation Using Y e a s t s - F r e e Cells . . . . . . . . . . . . . . . 1 Fed-Batch Process . . . . . . . . . . . . . .

8 References . . . . . . . . . . . . . . . . . . . . . . . . . 34 36 36 37 37 41 41 41 42 47 47 49 49 52 54 55 55 55 56 58 L-ephedrine is widely used in pharmaceutical preparations as a decongestant and anti-asthmatic compound. ), chemical synthesis involving resolution of a racemic mixture, or by biotransformation of benzaldehyde using various yeasts. In the present review, recent significant improvements in the microbial biotransformation are assessed for both fed-batch and continuous processes using free and immobilised yeasts.

Previous studies have reported concentrations of 10-12 g 1-1 L-PAC in batch culture with free cells [20, 21] and with immobilised yeast [22, 23]. The addition of cyclodextrins (particularly fl-cyclodextrin) was shown to enhance L-PAC production with immobilised Saccharomyces cerevisiae [24] although again maximal concentrations of 12 g 1-1 L-PAC were reported. Evaluation of a large number of yeast strains by Netrval and Vojtisek [19] identified strains of Saccharomyces, Candida and Hansenula sp.

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