By David C. Demirjian, Pratik C. Shah (auth.), Prof. Dr. Wolf-Dieter Fessner, A. Archelas, D. C. Demirjian, R. Furstoss, H. Griengl, K. -E. Jaeger, E. Morís-Varas, R. Öhrlein, M. T. Reetz, J.-L. Reymond, M. Schmidt, S. Servi, P. C. Shah, W. Tischer, F. Wedek
Catalytic tactics are sincerely the main not pricey capability to influence selective methods in natural synthesis. For the guidance of enantiomerically natural compounds, the usage of enzymes is very appealing due to excessive selectivities and delicate, environmentally benign response stipulations. making the most of advances in molecular biology, targeted new enzymes are actually with ease obtainable in volume with homes which are amenable to amendment on call for. This quantity brings jointly major members from the vanguard of this fascinating expertise. of their authoritative and well timed rewies they conceal the cutting-edge of biocatalysis from the invention of novel enzymes - through sleek screening, evolutionary or immunological methods - via immobilization innovations for technical tactics, to their use within the uneven synthesis of significant goal compounds. therefore, numerous chapters illustrate the bogus power of lately rising biocatalysts within the phospholipid, epoxide, cyanohydrin, and oligosaccharide fields.
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Extra resources for Biocatalysis - From Discovery to Application
1 Error-Prone PCR . . . . . . . . . . . . 2 Bacterial Mutator Strains . . . . . . . . . . 5 Directed Enzyme Evolution by Recombinative Methods . 1 DNA Shuffling . . . . . . . . . . . . . 2 Staggered Extension Process . . . . . . . . . 6 Gene Expression Systems . . . . . . . . . . 7 Selection and Screening . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36 37 38 39 39 39 40 40 42 42 43 3 Applications of Directed Evolution Methods .
The resulting variants were screened by the same procedure, leading to the identification of a new mutant exhibiting similar activity in aqueous buffer, but higher activity in the presence of DMF. Specifically, it turned out to be 38 times more active than wild-type subtilisin E in 85% DMF. T. -E. Jaeger of the enzyme. Although the information obtained by sequencing was not actually exploited in the efforts to generate higher enzyme activity, it was of interest with respect to structure/activity relations.
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