By S. Isaacs, H. Søeberg (auth.), Jan F. M. Van Impe, Peter A. Vanrolleghem, Dirk M. Iserentant (eds.)
This ebook is the outgrowth of the COMETT II direction on complex Instru mentation, info Interpretation, and regulate of Biotechnological methods geared up by way of the Katholieke Universiteit Leuven and the Universiteit Gent, and held at Gent, Belgium, October 1994. The editors of the current quantity have been very lucky to discover all invited audio system ready to write down cutting-edge expositions in accordance with their lec tures. designated thank you are because of them all. the result's an account of contemporary advances in instrumentation, facts interpretation, and version dependent op timization and regulate of bioprocesses. For somebody attracted to this emerg ing box, this article is of worth and gives finished studies in addition to new and significant traits and instructions for the long run, stimulated and illustrated via a wealth of functions. The typesetting of all this fabric represented a major quantity of labor. i'm such a lot thankful to my spouse, Myriam Uyttendaele, and to Kurt Gheys, who did many of the proof-reading. Their efforts have elevated much the uniformity standard and presentation of different manuscripts. Many thank you additionally to the co-editors, for his or her persevered aid. Kluwer educational Publishers is gratefully said for publishing this booklet, hence contributing to the move of the most recent study effects into huge scale business applications.
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Additional resources for Advanced Instrumentation, Data Interpretation, and Control of Biotechnological Processes
Although the BM will measure conductances up to 20 mS it begins to lose accuracy at measuring capacitances (and hence biomass) at levels above about 10 mS in low range and about 18 mS in high range. Thus when setting up a BM for a new fermentation one should first try measurements on the fermentation broth in low range. If the broth's conductance is above 10 mS then one must switch to high range. 4 Summary (a) The biomass monitor (BM) measures biomass by measuring the capacitance of the cell suspension.
7 g ammoniumchloride (Merck 1145) plus 10 g sodium tetraborate decahydrate (Merck 6308) plus 1 g EDTA plus 0. 7 mL copper sulfate solution in 1liter deionized water. R6 10 g sulfanilamide (Merck 11799) plus 52 mL concentrated HCJ in 1 liter deionized water. 0 g N-(1-naphthyl)-ethylene-diammonium-dichloride (Sigma N9125) in 1 liter deionized water (lasts approximately one to two weeks). Copper sulfate stock solution: 10 g copper sulfate (Merck 2790) in 1 liter deionized water . 82 g ammoniumchloride (Merck 1145) in 1 liter deionized water.
Originally, the system was implemented using a purely sequential measurement order, where a measurement cycle consisted of five FIA cycles performed in the sequence: standard solution, SPl, SP2, SP3 and SP4. At some point in time carry-over began to occur, partly due to configurational changes which affected flow rates and holdup volumes and partly due to 1. Flow Injection Analysis 34 other causes of carry-over described below. However, this went completely unnoticed until the currently employed nonsequential measurement cycle (standard solution, SP3, SP1, SP3, SP2, SP3, SP4, SP3) was implemented.