By Gerard Aihaud
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Additional info for Adipose Tissue Protocols (Methods in Molecular Biology Vol 155)
Light Microscopy Light microscopy (LM) allows study of the anatomy of the adipose organ. For instance, it permits investigation of its microscopic organization (possible lobular subdivision) and the arrangement in the tissue of vessels, nerves, and main cell types (1). 5), but the identification of other cell types, such as mast cells (particularly frequent in brown tissue), histiocytes (particularly frequent in white tissue, especially in fasting animals and in animals under diet restrictions), and inflammatory cells, requires magnification of at least ×40–100.
Nutr. Metab. Care 2, 101–104. 87. Björntorp, P. (1997) Hormonal control of regional fat distribution. Human Reprod. 12, 21–25. 88. Lafontan, M. and Berlan, M. (1993) Fat cell adrenergic recptors and the control of white and brown fat cell funtion. J. Lipid Res. 34, 1057–1091. Morphologic Techniques for BAT and WAT 21 2 Morphologic Techniques for the Study of Brown Adipose Tissue and White Adipose Tissue Saverio Cinti, M. 1. Light Microscopy Light microscopy (LM) allows study of the anatomy of the adipose organ.
1. 2. ) for tissue samples processed for traditional TEM. The authors perform these procedures on primary cultures of white and brown adipocytes, using the culture support itself as container, in which the various substances envisaged by the protocol are 42 Cinti et al. added. , Leighton tubes). After the identification of the areas containing the most significant cells, embedding is performed by tipping gelatin capsules filled with absolute resin onto them, in order as to include only the areas of interest.